Researcher
dr.ir. Jeroen Corver
Curriculum vitae
After my obtaining my MSc in Plant pathology at the University of Wageningen (1993), I did my PhD at the University of Groningen on Membrane fusion activity of Semliki forest virus, supervised by Prof. Dr. Jan Wilschut. During my PhD, I spent some time at the ETH in Zürich. In 1998, I moved to the California Institute of Technology (Caltech), to work as a postdoc on flaviviruses replication and assembly under supervision of Prof. Dr. James Strauss and collaborated with Profs Michael Rossmann and Richard Kuhn at Purdue University. Upon my return to the Netherlands in 2002, I started working in the LUMC on the spike proteins of hepatitis C virus and SARS coronavirus, in the group led by Prof. Dr. Willy Spaan. In 2008 I shifted towards bacteria, by working on the gut pathogen Clostridioides difficile, in the group of Prof. Dr. Ed Kuijper. In collaboration with Dr. Paul Hensbergen at the Center for proteomics and Metabolomics at the LUMC I now mainly work on C. difficile enzymes and their role in the regulation of motility and stress response of this notorious pathogen.
Research
My current work focusses on molecular processes in C. difficile and how they influence phenotypes and virulence factors. We zoom in on proteases and enzymes involved in the modifications of flagella. We also study homologous enzymes in other bacteria, including pathogens (for instance Pseudomonas aeruginosa). The work includes recombinant expression of bacterial enzymes, biochemical characterization (developing assays to measure activity) of these enzymes and genetic manipulation of C. difficile to study the role of these enzymes in the bacteria. Furthermore, we study gene-regulation and protein topology in C. difficile, focusing on processes that regulate virulence and survival.
Publications
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Proteolytic activity of surface-exposed HtrA determines its expression level and is needed to survive acidic conditions in Clostridioides difficile (2024).
J. Corver, B. Claushuis, T.M. Shamorkina, A.H. de Ru, M.M. van Leeuwen, P.J. Hensbergen, W.K. Smits
Mol. Microbiol. 122:413-428
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Non-prime- and prime-side profiling of Pro-Pro endopeptidase specificity using synthetic combinatorial peptide libraries and mass spectrometry (2024).
B. Claushuis, R.A. Cordfunke, A.H. de Ru, J. van Angeren, U. Baumann, P.A. van Veelen, M. Wuhrer, J. Corver, P.J. Hensbergen
FEBS journal 291: 3820-3838
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The C-Terminal Domain of Clostridioides difficile TcdC Is Exposed on the Bacterial Cell Surface (2020).
A.M. Oliveira Paiva, L. de Jong, A.H. Friggen, W.K. Smits, J. Corver.
J. Bacteriology 202: e00771-19
Groups:
Experimental Bacteriology
